Malheur Experiment Station
Oregon State University
Information for Sustainable Agriculture


Sugar Beet Variety TRialS 2007



Clinton C. Shock, Lamont D. Saunders, and Eric P. Eldredge

Malheur Experiment Station

Oregon State University

Ontario, OR



Introduction

The sugar beet industry in southern Idaho and eastern Oregon, in cooperation with Oregon State University, tests conventional and transgenic sugar beet varieties at multiple locations each year to identify varieties with high sugar yield and root quality. A seed advisory committee evaluates the data each year to select the best varieties for sugar production. This report provides the agronomic practices and results for the Malheur Experiment Station location of the 2007 trials.


Methods

Sugar beet varieties were entered by ACH Seeds, Betaseed, Hilleshog/Syngenta, Holly Hybrids, and Seedex in 2007. Thirty varieties were tested in the conventional trial, and 33 varieties were tested in the transgenic trial. In this report, conventional varieties are varieties that are not transgenic, while the transgenic varieties have not yet been released as commercial varieties for sale to the area growers. Seed was organized by Amalgamated Sugar Company, Paul, Idaho.

The 2007 sugar beet trials were grown on Greenleaf silt loam soil where winter wheat was the previous crop. A soil test taken on September 18, 2006, showed pH 7.7, 1.39 percent organic matter, 200 lb available nitrogen (N)/acre in the top 2 ft of soil, 24 ppm extractable phosphorus (P), 436 ppm exchangeable potassium (K), 8 ppm sulfate (SO4), 472 ppm magnesium (Mg), 83 ppm sodium (Na), 2.4 ppm zinc (Zn), 1.4 ppm copper (Cu), 10 ppm manganese (Mn), 15 ppm iron (Fe), and 0.8 ppm boron (B). The grain stubble was chopped and the field was irrigated and disked, and 200 lb sulfur (S)/acre and 2 lb B/acre fertilizer were applied on October 21. The field was deep ripped, plowed, and ground hogged before Telone® C17 at 15 gal/acre was injected on November 17 and the field was bedded in 22-inch rows.

On March 29, 2007, the bed tops were dragged off with a bed harrow. On March 30, Nortron® was applied at 6 pt/acre and incorporated using the bed harrow on the half of the field where the conventional trial would be planted. This was the first trial of transgenic glyphosate-resistant sugar beet varieties at Malheur Experiment Station. Both the conventional trial and the transgenic trial were planted on April 2. Seed for the 30 varieties tested in the conventional trial, and 33 varieties in the transgenic trial was organized by Amalgamated Sugar Company. Seeds were planted using John Deere model 71 flexi-planter units with double-disc furrow openers and cone seeders fed from a spinner divider that uniformly distributed the seed. Plots of each variety were 4 rows wide (22-inch row spacing) by 23 ft long, with a 3-ft alley separating each tier of plots. The seeding rate was 8 viable seed/ft of row. Each entry was replicated eight times in a randomized complete block design.

On April 4, Counter® 15G was applied in a band over the row at 7.4 lb/acre. The first irrigation was applied on April 11 for 24 hours. The field was furrow irrigated with surge irrigation from gated pipe, using a Waterman LVC-5 surge valve (Waterman Ind. Inc., Exeter, CA). Soil moisture was monitored using seven Watermark soil moisture sensors Model 200SS connected to an Irrometer Monitor (Irrometer Co. Inc., Riverside, CA) and another set of six Watermark soil moisture sensors Model 200SS connected to an AM400 Hansen datalogger (M.K. Hansen Co., Wenatchee, WA) soil moisture was maintained at a soil water tension wetter than 70 centibars (cb) at 10-inch depth in the beet row.

Beets had emerged by April 14, and were still mostly in the cotyledon stage by April 26. The conventional trial was sprayed with Progress® at 1.5 pt/acre plus Upbeet® at 0.5 oz/acre on April 27. The transgenic trial was sprayed with Roundup® WeatherMAX at 22 oz/acre on April 27. On May 3 the beets in the conventional trial were mostly in the 4- to 6-leaf stage, and in the transgenic trial were mostly in the 2- to 4-leaf stage. Alleys were hoed on May 4. Seedlings were thinned by hand to 1 plant per 7 inches on May 10 and 11. On May 11, urea was sidedressed to supply 173 lb N/acre.

The field was sidedressed with Temik® 15G at 10 lb/acre on May 14 to control sugar beet root maggot, and recorrugated. The field was irrigated for 24 hours on May 14 to move the insecticide with the wetting front into the sugar beet seedlings' root zone. On May 20, the conventional trial was sprayed with Progress at 4 pt/acre, Upbeet at 1 oz/acre, Stinger® at 0.5 pt/acre, and Treflan® at 1.5 pint/acre, and the transgenic trial was sprayed with Roundup WeatherMAX at 22 oz/acre. The field was cultivated on May 23 and irrigated on May 29. The first irrigation in the wheel and center furrows was applied on June 4.

The first petiole test, taken on June 8, showed N low at 6,336 ppm, when the sufficiency level was 11,220 ppm; phosphate (P2O5) was at the low end of the sufficiency range with 0.27 ppm when the range was 0.25 to 0.7 ppm; SO4 was low at 0.10 ppm when the sufficiency range was from 0.17 to 0.5 ppm; Mg was marginally sufficient at 0.29 ppm in the sufficiency range of 0.20 to 0.70 ppm; Zn was 19 ppm when the sufficiency range was 17 to 35 ppm; Cu was 9 ppm when the sufficiency range was 5 to 25 ppm; B was slightly low at 22 ppm when the sufficiency range was 23 to 80 ppm, and all other nutrients were sufficient.

Headline® fungicide at 12 oz/acre plus 9-28-3 to supply 1 lb P2O5/acre, 0.2 lb Zn/acre, and 0.2 lb Cu/acre were applied by aerial applicator on June 16 to amend nutrients and prevent powdery mildew. With the June 18 irrigation, Epsom salt to supply 5 lb Mg/acre and 6.6 lb SO4/acre, ammonium thiosulfate (thio-sul) to supply 3.4 lb SO4 and 2.6 lb N/acre, solution 32 (UAN) to supply 17.4 lb N/acre, and boron solution to supply 0.2 lb B/acre were applied in the irrigation water.

The second petiole test, taken June 25, showed that N was low at 6,400 ppm when sufficiency was 10,050 ppm and B was low at 22 ppm when sufficiency was 23 ppm. The conventional trial was hand weeded on June 25 in a total of 15 hours, and the transgenic trial was not hand weeded. UAN to supply 20 lb N/acre was applied in the irrigation on June 26. Enable® fungicide at 8 oz/acre with 4 lb S/acre and 0.1 lb B/acre was applied by aerial applicator on June 30. The transgenic trial was sprayed with Roundup WeatherMAX at 22 oz/acre in a spray volume of 28 gal/acre on July 2.

The third petiole test, taken July 9, showed deficiencies in N, SO4, Mg, and Zn. Thio-sul, UAN, Epsom salt and che-Zinc were applied in the irrigation water on July 12 to supply 20 lb N/acre, 27 lb SO4/acre, 1.5 lb Mg/acre, and 0.25 lb Zn/acre. An aerial application of Headline fungicide at 12 oz/acre was done on July 15 and included 4 lb S/acre, 0.25 lb Mg/acre, and 0.2 lb Zn/acre.

TopsinM® fungicide at 1 lb/acre plus 4 lb S/acre, 0.25 lb Mg/acre, 0.2 lb Zn/acre, and 0.1 lb Cu/acre were applied by aerial applicator on August 1 to amend nutrients and prevent powdery mildew. The trials were hand weeded on August 2, taking a total of 8 hours on the conventional trial and 7 hours on the transgenic trial.

A fourth petiole test taken on August 14 showed nitrate high at 14,620 ppm when sufficiency was 6,150 ppm, and deficiencies in P2O5, S, and Zn. Several species of lepidoptera larvae began feeding on the foliage in mid-August and were controlled with an aerial application on August 23 of Asana® at 9.6 oz/acre. The application included Enable at 8 oz/acre and S at 4 lb/acre because powdery mildew was seen on some young leaves and P2O5 at 10 lb/acre and Zn at 0.25 lb/acre to correct nutrient deficiencies.

Bolted beets were counted and removed from plots in the transgenic trial beginning on June 25, and again on July 2, July 9, July 23, and August 15. 'HM9047RR' had a total of 39 bolters, 'HM9076RR' had 28, 'HM9058RR' had 25, and 'HM9040RR' had 2 bolters.

The transgenic sugar beets were harvested October 15 and the conventional trial was harvested October 16. The foliage was flailed and the crowns were removed with rotating disks. All sugar beets in the center two rows of each plot were dug with a two-row wheel-lifter harvester and weighed, and two eight-beet samples were taken from each plot. Samples were hauled each day to the Snake River Sugar factory for laboratory analysis of percent sucrose, nitrate concentration, and conductivity.

The root weight data were examined for outliers as is customary for calculations of sugar beet variety data in these trials. Observations more than two standard deviations from the mean for each variety were deleted. Sugar sample data were checked for errors in sugar percentages and conductivity. Any erroneous sample readings were deleted from the data set.

The weight of sugar beets from each plot was multiplied by 0.90 to estimate tare. Sugar concentrations were "factored" by multiplying measured sucrose by 0.98 to estimate the sugar that would have been lost to respiration if the beets had been stored in a pile. The data for each plot with two samples were averaged for analysis. The percent extraction was calculated using the formula:

Ext = 250 + [(1,255.2 * Cond) - (15,000 * Sug) - 6,185] / Sug *(98.66 - 7.845 * Cond)

where Ext is percent extraction, Cond is the electrical conductivity in mmho, and Sug is the sucrose concentration in percent.

Variety differences in yield, sucrose content, conductivity, percent extraction, and estimated recoverable sugar were calculated using least-squares means analysis. The varieties are listed in the tables in descending order of estimated recoverable sugar.

Reports of previous years' Oregon State University variety trials are available online at www.cropinfo.net.



Results

The 1.5-acre conventional beet trials required a total of 23 hours of weeding to remain weed free at harvest while the 1.5-acre transgenic trial required only 7 hours.

Variety results were grouped by estimated recoverable sugar for the conventional trial (Table 1) and the transgenic trial (Table 2). The root weights were tared 10 percent, as explained above.

Root yield in the conventional trial averaged 45.18 tared ton/acre and 17.9 percent sugar content (Table 1). Varieties with the highest root yield in the conventional trial included 'Beta 4720R' (49.54 ton/acre), 'Crystal 333R', 'Crystal 316R', 'HH Meridian R', 'HH 06HX623 R', 'Crystal 597R', 'HM 2996Rz', 'Crystal 505R', 'HH Pomerelle R', 'HM 2993Rz', 'HH 06HX620R', and 'Beta 5181N'. Varieties among those with the highest recoverable sugar per acre included Beta 4720R (14,189 lb/acre), Crystal 597R, Crystal 333R, Beta 5181N, Crystal 316R, HH Meridian R, Crystal 505R, 'HM 2996Rz', HH 06HX623 R, HH 06HX620 R, HM 2993Rz, and HH Pomerelle R.

Root yield in the transgenic trial averaged 47.19 tared ton/acre and 17.67 percent sugar content (Table 2). Varieties with the highest root yield in the transgenic trial included 'BTS27RR10' (53.17 ton/acre), 'BTS 26RR18', 'HM9036RR', 'HM 9077RR', 'BTS26RR14', 'Crystal RR929', 'BTS 27RR20', 'BTS 25RR07', and 'BTS 25RR05'.

Varieties with the highest recoverable sugar per acre included BTS 27RR10 (15,880 lb/acre), BTS 26RR18, Crystal RR929, BTS 25RR05, HM 9036RR, HM 9077RR, BTS 27RR20, BTS 26RR14, and BTS 25RR07.







Table 1. Performance of conventional sugar beet varieties, Oregon State University, Malheur Experiment Station, Ontario, OR, 2007.

Variety

Root

yield

Sugar

content

Gross

sugar

Conduc-

tivity

Extrac-

tion

Estimated recoverable sugar


ton/acre

%

lb/acre

mmhos

%

lb/ton

lb/acre

rankinga

Beta 4720R

49.54

16.81

16651

0.729

85.23

286.6

14189

a

Crystal 597R

47.45

17.59

16696

0.765

84.91

298.7

14177

a

Crystal 333R

49.01

16.79

16448

0.756

84.87

284.9

13960

ab

Beta 5181N

45.03

17.93

16147

0.658

86.37

309.7

13943

abc

Crystal 316R

48.99

16.81

16461

0.778

84.59

284.4

13922

abc

HH Meridian R

48.11

17.10

16453

0.796

84.40

288.7

13890

abc

Crystal 505R

46.49

17.46

16216

0.763

84.90

296.4

13760

abc

HM 2996Rz

47.45

17.08

16200

0.771

84.73

289.5

13729

abc

HH 06HX623 R

47.82

16.95

16205

0.791

84.45

286.2

13686

a-d

HH 06HX620 R

45.15

17.57

15859

0.700

85.76

301.4

13599

a-e

HM 2993Rz

45.92

17.15

15750

0.737

85.19

292.2

13419

a-f

HH Pomerelle R

46.08

17.11

15769

0.744

85.09

291.2

13417

a-f

HM 2999Rz

45.50

17.14

15595

0.773

84.72

290.5

13213

b-g

Crystal 217R

46.25

16.86

15601

0.779

84.58

285.2

13199

b-g

HH Acclaim R

47.01

16.51

15520

0.756

84.82

280.1

13165

b-g

HM 2984Rz

47.42

16.24

15400

0.703

85.45

277.6

13157

b-g

SX Raptor Rz

45.03

17.22

15508

0.769

84.78

292.0

13148

c-g

SX 1524 Rz

44.66

17.10

15264

0.779

84.63

289.3

12917

d-h

Beta 4773R

44.89

17.00

15269

0.806

84.26

286.6

12866

e-i

HH 06HX626 R

40.71

18.12

14751

0.600

87.15

315.8

12851

e-i

HH Phoenix R

46.28

16.32

15087

0.777

84.50

275.9

12743

f-j

HH Eagle R

44.98

16.58

14924

0.731

85.15

282.4

12711

f-j

HM 2988Rz

41.80

17.76

14846

0.739

85.25

302.7

12659

f-j

HM 1339Rz

43.29

17.23

14900

0.759

84.91

292.6

12655

f-j

SX Cascade

47.91

15.17

14535

0.595

86.60

262.7

12586

g-j

Beta 4199R

41.17

17.52

14436

0.826

84.09

294.8

12141

h-k

HH Condor R

40.33

17.38

14030

0.678

86.01

299.0

12065

ijk

Beta 4910R

40.38

17.53

14152

0.794

84.52

296.3

11961

jk

Beta 4490R

39.37

17.68

13911

0.796

84.51

298.8

11755

k

Beta 4023R

41.02

16.97

13923

0.861

83.51

283.5

11629

k

LSD (0.05)

2.71

0.33

940

0.065

0.88

7.1

811


Grand Mean:

45.18

17.09

15422

0.751

84.99

290.5

13108


aEstimated recoverable sugar amounts followed by different letters are significantly different. If the same letter is shared, the amounts are not statistically different.







Table 2. Performance of transgenic sugar beet varieties, Oregon State University, Malheur Experiment Station, Ontario, OR, 2007.

Variety

Root

yield

Sugar

content

Gross

sugar

Conduc-

tivity

Extrac-

tion

Estimated recoverable sugar


ton/acre

%

lb/acre

mmhos

%

lb/ton

lb/acre

rankinga

BTS 27RR10

53.17

17.05

18131

0.551

87.58

298.7

15880

a

BTS 26RR18

52.27

17.34

18125

0.591

87.12

302.2

15790

ab

Crystal RR929

48.61

18.24

17732

0.599

87.19

318.1

15461

abc

BTS 25RR05

46.98

18.42

17312

0.614

87.03

320.7

15066

a-d

HM 9036RR

50.93

16.97

17271

0.596

86.99

295.3

15025

a-d

HM 9077RR

49.83

17.42

17356

0.639

86.52

301.4

15017

a-d

BTS 27RR20

48.58

17.68

17177

0.598

87.10

308.1

14956

a-e

BTS 26RR14

49.80

17.21

17134

0.577

87.28

300.5

14955

a-e

BTS 25RR07

48.23

17.83

17197

0.628

86.74

309.4

14920

a-f

Crystal RR919

48.58

17.45

16946

0.551

87.66

306.0

14854

b-g

BTS 26RR13

48.49

17.53

16979

0.584

87.25

305.8

14817

b-h

Crystal RR968

48.08

17.54

16867

0.610

86.92

304.9

14664

c-i

BTS 26RR11

48.43

17.56

16997

0.666

86.20

302.7

14650

c-i

HM 9058RR

47.13

17.72

16696

0.573

87.43

310.0

14598

c-i

BTS 26RR15

45.46

18.03

16393

0.482

88.64

319.6

14532

c-j

HM 9040RR

48.58

17.24

16734

0.647

86.39

297.9

14456

d-k

HH SV702RR

46.81

17.60

16485

0.578

87.34

307.4

14401

d-k

BTS 26RR17

48.32

17.26

16665

0.664

86.16

297.5

14364

d-k

HH SV701RR

46.75

17.66

16497

0.600

87.07

307.5

14360

d-k

HM 9047RR

45.38

17.83

16191

0.559

87.62

312.5

14191

d-k

HM 90706RR

46.95

17.60

16503

0.688

85.90

302.3

14178

d-k

Crystal RR989

45.88

18.01

16513

0.706

85.75

309.0

14162

d-k

HM 90701RR

45.38

17.84

16185

0.573

87.45

311.9

14151

d-k

HM 9006RR

45.79

17.73

16230

0.618

86.86

308.1

14096

d-k

HH B05G26001RR

44.89

17.86

16041

0.589

87.25

311.6

13995

e-k

HM 9008RR

45.30

17.77

16100

0.617

86.87

308.7

13985

e-k

HM 9076RR

45.09

17.80

16016

0.604

87.04

309.8

13947

f-k

HM 9052RR

45.06

17.89

16100

0.646

86.52

309.5

13919

g-k

Crystal RR985

45.21

17.63

15939

0.619

86.81

306.0

13840

h-k

Crystal RR966

44.34

18.20

16132

0.721

85.59

311.4

13810

ijk

HM 9009RR

45.41

17.41

15793

0.621

86.75

302.2

13700

ijk

HM 9041RR

44.28

17.74

15690

0.644

86.51

307.0

13580

jk

BTS 25RR06

42.86

17.97

15386

0.557

87.68

315.1

13493

k

LSD (0.05)

3.10

0.37

1107

0.058

0.76

7.5

989


Grand Mean:

47.19

17.67

16655

0.610

86.94

307.2

14481


aEstimated recoverable sugar amounts followed by different letters are significantly different. If the same letter is shared, the amounts are not statistically different.

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